Systematic Identification and Targeted Analysis of Intraluminal and Peripheral Thylakoid Proteins

In higher plants, thylakoids are the site of photosynthetic light reactions, sites of light capture, photosynthetic electron transfer, proton translocation, and ATP synthesis. Morphologically, thylakoids can be divided into granum thylakoid and stroma thylakoid, which are significantly different in protein composition. Most of the proteins and protein complexes involved in photosynthesis are localized in the thylakoid membrane and thylakoid lumen. In addition to the more detailed study of major photosynthetic complexes such as photosystem I (PS I), photosystem Ⅱ (PS Ⅱ), cytochrome b6f complex (Cytb6f) and ATP synthase. In recent years, a variety of thylakoid membrane proteins have been identified through the study of thylakoid proteomics technology, which play an important role in the processes of biosynthesis and cofactor attachment, protein insertion into membrane, protein folding and degradation.

Fig. 1. Proteins targeting into thylakoid membranes through distinct pathways. (Zhu D, et al., 2022)

Services

Proteomics bridges the gap between genome sequence information and the actual protein populations within a particular tissue, cell, or cell lumen. Research suggests that more proteins may be located in or on the thylakoid membrane. Over the years, the team at Lifeasible has extensive experience in the systematic identification and targeted analysis of thylakoid proteins in the chloroplast lumen and periphery. We are committed to further characterizing the thylakoid luminal proteome through a unique combination of experiments and localization predictions. Here, we not only help you identify luminal proteins with known functional domains, such as proteins involved in photosynthetic electron transport, ATP synthesis and photorespiration, proteins involved in protein folding, processing and hydrolysis, proteins involved directly or indirectly in antioxidant defense. In addition, many luminal proteins of unknown function were identified by systematic searches. We employed the following strategies to systematically identify proteins in plant thylakoids:

· Two-dimensional electrophoresis, mass spectrometry, N-terminal Edman sequencing and other methods were used to systematically analyze the soluble and peripheral proteins in various plant thylakoids, and conduct database search.

· We combined experimental proteomic analysis with a genome-wide predictive screen to characterize the protein content of the thylakoid lumen of plant-mimicking chloroplasts.

· We thoroughly validate the gene annotation of all experimentally identified luminal proteins and demonstrate a general strategy for re-annotation.

· Genome-wide mapping predicts and analyzes the luminal proteome of plants.

Competitive Advantages

• Using multiple strategies to systematically and comprehensively characterize the thylakoid proteome.
• Information containing the structure and function of protein complexes and the three-dimensional structure of the thylakoid membranes that house these proteins or complexes.
• A team with rich experience in chloroplast protein research.
• A unique combination of experimentation and location prediction.
• The analysis results are fast and reliable.
• Gain a deeper understanding of the function of chloroplast thylakoids and their metabolic laws.

Lifeasible can meet the needs of customers on time and on budget through a wide range of strategies for analyzing chloroplast proteome. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

References

1. Peltier J B, Friso G, Kalume D E, et al. (2000) Proteomics of the chloroplast: systematic identification and targeting analysis of lumenal and peripheral thylakoid proteins[J]. The Plant Cell. 12(3): 319-341.
2. Zhu D, Xiong H, Wu J, et al. (2022) Protein Targeting Into the Thylakoid Membrane Through Different Pathways[J]. Frontiers in Physiology. 2396.