Study on the Functional Pattern of Chloroplast Proteins

Chloroplasts are organelles that carry out photosynthesis, which is the source of matter and energy for human survival. However, the chloroplast needs thousands of different proteins to function, which are imported into the chloroplast through specialized machinery called the TOC complex. Furthermore, the chloroplast is composed of several sub-organelle compartments, including the envelope, stroma, and thylakoid membranes. There is a link between the functions of these compartments and their constituent proteins. As a model organism, Arabidopsis plays an important role in chloroplast engineering research. With the completion of the Arabidopsis genome sequencing and the development of computer software, it is speculated that there are about 4255 nuclear genome-encoded chloroplast proteins, and a search for the functional annotation of these proteins in the TAIR database found that about 25% of the protein functions are unknown. Researchers have successfully used mass spectrometry (MS)-based proteomics to further analyze the functions of these component proteins.

Fig. 1. Plastid protein import and control of the import machinery by the ubiquitin-proteasome system. (Richardson L G L, et al., 2017)

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It is estimated that higher plants have a total of about 21,000 to 25,000 proteins, of which chloroplast proteins account for 10% to 20%, which fully proves the importance of chloroplasts in plant cells. These proteins play an important role in participating in specific biochemical synthesis and metabolic processes in the chloroplast. Over the years, the team at Lifeasible has extensive experience in studying the functional analysis of chloroplast proteins. Our scientists are committed to developing a multi-scale integrated approach to assess the function of chloroplast proteins throughout the organism and to reveal their structures at the atomic level. In addition, we can help you identify many unknown chloroplast kinases and protein phosphatases from chloroplast phosphorylation analysis to characterize post-translational modifications of proteins. According to the chloroplast structural composition and biochemical properties, we focus on the analysis of the functions of the following chloroplast proteins:

• Chloroplast Envelope Proteins
  We employed proteomic techniques to study chloroplast membrane proteins in Arabidopsis, spinach, and green algae. Identification of purified chloroplast membrane proteins will help you identify novel envelope proteins and predict their function.
• Chloroplast Matrix Proteins
  We co-identified the matrix protein and predicted its function by further isolating matrix protein components from intact chloroplasts, combined with native electrophoresis and two-dimensional SDS-PAGE analysis.
• Chloroplast Thylakoid Proteins
  We used proteomic techniques to analyze the proteins in chloroplast thylakoid of various plants, and combined with computer to predict the function of each protein in the chloroplast thylakoid cavity.

Competitive Advantages

• Our team has extensive knowledge and research experience in chloroplast protein purification and functional analysis.
• Providing new insights into intracellular protein trafficking and sorting.
• Chloroplast membrane proteomes of different model plants were analyzed under different conditions.
• Contributing to an in-depth understanding of chloroplast development and biological function.
• A multi-scale, comprehensive approach.

Lifeasible can meet the needs of customers on time and on budget through a wide range of strategies for analyzing chloroplast proteome. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

Reference

1. Richardson L G L, Singhal R, Schnell D J. (2017) The integration of chloroplast protein targeting with plant developmental and stress responses[J]. BMC biology. 15(1): 1-10.