Production of Chloroplast-Derived Human Somatotropin

Introduction

Growth hormone (GH) or somatotropin is a peptide hormone that plays an important role in stimulating growth, cell reproduction and cell regeneration in humans and other animals. Human growth hormone deficiency often occurs in children and adults. Human somatotropin (hST) is a therapeutic protein for the treatment of hypopituitarism, Turner syndrome, and chronic renal failure. The development of recombinant DNA technology has facilitated the safe and mass production of recombinant human somatotropin in various heterologous systems. Production of hST in bacterial and fungal expression systems is relatively inexpensive, but is limited by the absence of post-translational modifications. In addition, the production of hST in mammalian expression systems is also limited due to the low yield of the protein and the high cost of production. Plants are a promising expression system for the production of recombinant proteins. Scientists have successfully expressed chimeric hST in transgenic tobacco chloroplasts, demonstrating that chloroplasts can efficiently fold hST proteins and form disulfide bonds.

Fig. 1. Therapeutic human somatotropin, and human growth hormone detection and analysis.

Solutions

Our engineers are passionate about chloroplast engineering to produce therapeutic proteins, for research to treat or prevent human or animal disease. Lifeasible is an expert in the field of recombinant protein research and development, with a variety of mature and comprehensive platforms and technologies, providing global customers with professional solutions for the production of chloroplast-derived human somatotropin proteins.

Growth hormone requires proper folding and disulfide bond formation. Here, we provide a plant chloroplast-based expression system to produce high-purity recombinant human growth hormone. Human growth hormone proteins with folded eukaryotic proteins and added disulfide bonds were developed by utilizing the chloroplast enzyme protein disulfide isomerase. In addition, we have developed an expression system based on the Tobacco Mosaic Virus (TMV) viral vector for high-yield production of human growth hormone. Our solution flow is roughly as follows:

(1) The hST gene was cloned into the TMV expression vector.
(2) The vector carrying the hST gene was introduced into the tobacco chloroplast genome by Agrobacterium transformation.
(3) Plant inoculation and inoculum preparation for large-scale production of hST.
(4) The expression of recombinant hST protein in plants was analyzed by PAGE and western blot.

Features of Our Solutions

• The level of hST produced in the chloroplast is as high as 7% of TSP.
• Expressed hST represents an active form of the same hGH that has been used for therapeutic purposes.
• Plants can produce large amounts of biomass in existing infrastructure such as in open field or greenhouse environments.
• Plant cells, like animal cells, can produce secreted proteins and undergo post-translational modifications.
• Plants can be used as production systems in different ways, transgenic methods and transient methods.
• Plant virus systems are characterized by high levels of recombinant protein expression.
• Short lead times, flexibility and fast mass production.

The chloroplast-transformed human somatotropin protein is safe and effective, also prevents and treats dwarfism, fractures, skin burns, bleeding ulcers and AIDS, etc. Lifeasible has extensive knowledge and experience in the development and production processes of the chloroplast-derived human somatotropin protein. Our mission is to provide customers with comprehensive, reliable, professional solutions to accelerate your research. If you are interested in our solutions for the production of chloroplast-derived human somatotropin, please contact us at any time.

References

1. Rabindran S, Stevenson N, Roy G, et al. (2009) Plant-produced human growth hormone shows biological activity in a rat model. Biotechnol Prog. 25(2): 530-534.
2. Suleiman A A, Ibrahim K M, Al-Shaibani A B. (2013) Production of Lettuce Edible Vaccine for Cholera Disease Using Chloroplast Genetic Engineering[J]. Journal of university of Anbar for Pure science. 7(1).