Analysis of Chloroplast Sulfoquinolosyl Diacylglycerols Biosynthesis

Lifeasible is committed to the development and research of chloroplast engineering. With years of experience in chloroplast lipidomics, With years of experience in chloroplast lipidomics, we are able to provide analysis of chloroplast sulfoquinolosyl diacylglycerols biosynthesis services to understand the role of sulfoquinolosyl diacylglycerols in chloroplast photosynthesis and plant stress response, precisely meeting customer requirements.

Introduction

Sulfatide sulfoquinolosyl diacylglycerol (SQDG) is an abundant sulfur-containing nonphospholipid glycerolipid, which exists on the photosynthetic membranes of plants and most photosynthetic bacteria, and is the only one on the photosynthetic membranes with a Negatively charged glycolipids. Its characteristic structural feature is the unique head group component, sulfoquinovose, a glucose derivative, in which the 6-hydroxyl group is replaced by a sulfonic acid group. SQDG is generally considered to be particularly relevant to the photosynthetic membranes of higher plants, mosses, ferns, algae and most photosynthetic bacteria. But the content of SQDG is also related to biological species, and there are certain differences in the content of SQDG in these plants. In the past 40 years, the synthesis of SQDG has been extensively studied, but its biosynthetic pathway is not fully understood. The existence of SQDG on the plastid membrane and the uniqueness of its structure have made many researchers interested in the role and synthesis of SQDG in the thylakoid membrane and even in photosynthesis.

Fig. 1. Sugar-nucleotide pathway hypothesis for SQDG biosynthesis. (Benning C, 1998)

Customized Services

The synthesis of sulfatide involves multiple metabolic pathways such as sugar metabolism, sulfate metabolism, and lipid metabolism. Among them, the source of sulfonic acid group donors in SQDG is the key to study its synthesis, and it is also a major constraint to the slow progress of SQDG synthesis. Lifeasible is committed to the analysis of chloroplast sulfoquinolosyl diacylglycerols biosynthesis, using ³⁵S isotope tracer to study the source of sulfonic acid groups in SQDG, biochemical analysis of SQDG synthase and identification of SQDG biosynthesis in photosynthetic bacteria and higher plants necessary for biosynthesis genes, so as to help you understand the role and synthetic pathways of sulfoquinolosyl diacylglycerols in thylakoid membranes and even in photosynthesis.

Thylakoid chromoprotein complexes always contain SQDG, which may stabilize or regulate their structure or function. Our team has extensive experience in studying chloroplast SQDG biosynthesis, and provides comprehensive customized services for the study of SQDG synthesis pathways and functions by combining biochemical and molecular biology techniques:

· Identification of genes essential for SQDG biosynthesis.
· Determine the precise location of SQDG synthase on the chloroplast envelope.
· Analysis of the interaction of several chromoprotein complexes (such as LHC Ⅱ and PSⅡ) with SQDG in higher plants.
· Analysis of the effect of deletion mutants of SQDG on plant growth and development under phosphorus-limiting conditions.
· Analysis of the role of SQDG in plant stress resistance.

Applications

• Providing a means to study the regulation of thylakoid membrane lipid composition at the lipid level.
• Developing biomedical applications of SQDG, such as anti-tumor, anti-viral properties, etc.
• Using advanced molecular techniques to study the synthesis and function of SQDG at the protein and gene levels.
• The synthetic pathway of SQDG was analyzed based on the knowledge of the synthetic pathway of UDP-SQ.

Lifeasible is committed to developing a variety of cutting-edge technologies to help customers analyze the role of chloroplast lipids in chloroplast biosynthesis and photosynthetic function. We are proud to regulate plant growth and development, as well as control their pests, through chloroplast protein modifications. If you are interested in our services, please do not hesitate to contact us for more information.

References

1. Benning C. (1998) Biosynthesis and function of the sulfolipid sulfoquinovosyl diacylglycerol[J]. Annual review of plant biology. 49(1): 53-75.
2. Aoki M, Tsuzuki M, Sato N. (2012) Involvement of sulfoquinovosyl diacylglycerol in DNA synthesis in Synechocystis sp. PCC 6803[J]. BMC Research Notes. 5(1): 1-8.