Particle Bombardment-Mediated Chloroplast Transformation

Introduction

Chloroplast genetic engineering of higher plants is a new field of plant genetic engineering development. As early as 1988, scientists succeeded in transforming chloroplasts with exogenous genes by particle bombardment using Chlamydomonas reinhardtii as material. Since then, this technique has been successfully used for the transformation of chloroplasts of a variety of higher plants. The particle bombardment method, also known as the gene gun. It is based on the principle of wrapping a complete plasmid vector on the surface of metal particles (such as gold powder and tungsten powder), bombarding various tissues and organs of the plant with the gene gun, and then continuously screening the recombinant chloroplasts. Continuously improve the homogenization level, and finally obtain the desired transgenic plants. Plant cell walls are generally impermeable to foreign DNA, so this method becomes a common method for genetic transformation of plants, and it is not limited to any particular species or plant cell.

Fig. 1. Plant transformation via particle bombardment. (Ozyigit II, et al., 2020)

Services

Particle bombardment is versatile and widely applicable, our technology in this area is quite mature. With the support of chloroplast genetic engineering platform, Lifeasible can use particle bombardment method to carry out high-quality chloroplast genetic transformation of a variety of plants, helping you easily realize the application of chloroplast genome transformation in agronomic traits and biopharmaceuticals. In addition, we can use this method to transform other plant tissues, including cell suspensions, callus, immature embryos, mature embryo parts, meristems, leaves, microspores, and pollen.

Our services are not only focused on nuclear transformation, but can successfully transform stable, plastid genes of your desire that are not targeted or disrupted by any other cellular components on the cell surface. In conclusion, our aim is to achieve efficient transformation of chloroplast genomes in plants based on particle bombardment and to analyze the efficacy of chloroplast transformation to produce higher levels of heterologous proteins. We will provide you with chloroplast transformation services by particle bombardment based on the following process.

(1) Coat the desired DNA construct with gold/tungsten particles.
(2) Immobilize DNA-coated microcarriers on macrocarriers.
(3) Correspondingly set rupture discs, baffles and macroscopic carriers in the barrel.
(4) Fix the chloroplast at the end of the gun chamber.
(5) Use gas force to bombard the chloroplasts with particles.
(6) Chloroplasts transformed with DNA constructs.

Advantages of Particle Bombardment

Since the equipment and chemicals used in this method are expensive, its cost is relatively high. In addition, chaotic consolidation patterns will result in relatively low throughput. But you don't need to worry about these, our professional engineers will provide you with the lowest cost and reliable results. This approach has been adopted by us due to its many advantages.

• Can transform many different tissues and cell types.
• No binary vector required.
• Can provide multiple plasmids with high co-transformation frequency.
• Simple conversion process.
• Ability to deliver proteins, RNA and RNP directly into plants.
• Ability to transform multiple genes at once within target cells.

Lifeasible has successfully constructed a chloroplast gene transformation platform, using a variety of strategies to achieve chloroplast engineering. With our team's extensive experience in the construction and transformation of chloroplast gene vectors, we are able to provide high-quality particle bombardment-based chloroplast transformation platforms to global customers. If you are interested in our services, please do not hesitate to contact us for more information.

References

1. Ozyigit II, Yucebilgili Kurtoglu K. (2020) Particle bombardment technology and its applications in plants. Mol Biol Rep. 47(12):9831-9847.
2. Cheng L, Li HP, Qu B, Huang T, et al. (2010) Chloroplast transformation of rapeseed (Brassica napus) by particle bombardment of cotyledons. Plant Cell Rep. 29(4):371-81.