Chloroplast Metabolomics Services

The diversity of metabolites produced by plants far exceeds those produced by animals and microorganisms, so chloroplast metabolomics has always been a research hotspot, involving areas including the analysis of changes in metabolites in chloroplasts, analysis of multiple metabolic pathways in chloroplasts, and metabolic comparative analysis of networks. As an expert in the field of chloroplast engineering, Lifeasible is committed to the research of chloroplast metabolomics, and provides reliable metabolomic analysis services for chloroplasts of various plants through biotechnology.

Introduction

With the rapid development of genomics and proteomics, metabolomics research has received more and more attention. Metabolomics is a research method that quantitatively analyzes all metabolites in an organism and finds the relative relationship between metabolites and physiological and pathological changes. It plays a key role in analyzing biological systems and gene functions. Plants collectively produce vast quantities of chemicals, far more than most other organisms produce. In addition, the chloroplast is an important organelle for photosynthesis in green plants and is the site of countless different chemical reactions. However, the chemical composition and biological activity of only a small fraction of metabolites in plants have been studied. People try to study the physiological activities and biosynthetic pathways of these metabolites through a variety of biotechnologies, and to identify key metabolic pathways and key enzymes and their associated genes.

Fig. 1. The typical pipeline of metabolomics research and detectable metabolites. (Saito K, et al., 2010)

Our Services

Our scientists have successfully built a multi-faceted, fully integrated strategy for chloroplast metabolomics analysis, from optimal sample extraction, metabolite isolation/detection/identification, automated data collection/processing/analysis, to final metabolite analysis quantify. We will choose the appropriate chloroplast metabolomics strategy for you based on your experimental purpose and sample:

· Off-Target Metabolomics Strategy
We compared the content of metabolites in different groups of samples in chloroplasts to find out the differences of metabolites. This strategy is widely used in differential metabolite search and screening, disease pathogenesis research, and drug treatment mechanism research.

· Target Metabolomics Strategy
We use LC-MS or GC-MS for targeted and specific detection and analysis of specific metabolite groups, ranging from highly polar compounds in the calvin cycle to semi-polar plant hormones and plant lipids several classes of compounds such as non-polar compounds.

Our services contribute to a deeper understanding of the complex interactive nature of plant metabolic networks and their responses to environmental and genetic changes, but will also provide comprehensive insights into plant phenotype and development, physiology, tissue properties, resistance, biodiversity. Our chloroplast metabolomics services include but are not limited to:

• Identification of Chloroplast Metabolites
• Expression Analysis of Chloroplast RNA Metabolism
• Qualitative and Quantitative Analysis of Chloroplast Metabolites
• Regulation of Chloroplast Metabolism

Methods for Chloroplast Metabolomics Research

Our dedicated metabolomics team has extensive experience in chloroplast metabolomic analysis. At the same time, we combine advanced technology with various analysis software or databases to provide you with comprehensive metabolomics data analysis services. The following detection techniques can be flexibly selected according to different experimental requirements:

• Chromatography-mass spectrometry (MS).
• Nuclear magnetic resonance (NMR).
• Fourier transform-infrared spectrometer (FT-IR).
• Gas chromatography (GC)-MS.
• Liquid chromatography (LC)-MS.
• Capillary electrophoresis (CE)-MS.

Lifeasible provides you with professionally and economically oriented chloroplast engineering service projects. Each project will be individually designed to meet our customers’ requirements and described in a detailed project report. Our customers have direct access to our experts and provide immediate feedback on any online inquiries. If you are interested in our services, please do not hesitate to contact us.

References

1. Saito K, Matsuda F. (2010) Metabolomics for functional genomics, systems biology, and biotechnology[J]. Annual review of plant biology. 61: 463-489.
2. Schauer N, Fernie A R. (2006) Plant metabolomics: towards biological function and mechanism[J]. Trends in plant science. 11(10): 508-516.