Expression Analysis of Chloroplast RNA Metabolism

RNA is the only macromolecule known to function simultaneously in the storage, transfer, and catalysis of genetic information, as an essential chemical intermediate in the development of life on earth. As the site of aerobic photosynthesis, the chloroplast is the most deeply studied plant organelle. Expression analysis of chloroplast genetic information revealed that the chloroplast gene expression system is far more complex than its cyanobacterial progenitors. This complexity is caused by changes in chloroplast RNA metabolism. Mature chloroplast RNA is produced by numerous nuclear-encoded proteins acquired or recruited during plant evolution, including additional RNA polymerases and sigma factors, and sequence-specific RNA maturation that promotes RNA splicing, editing, end formation, and translatability factor. Furthermore, many steps of chloroplast RNA metabolism are essential for gene expression. Therefore, studying the complexity of chloroplast RNA metabolic expression is of great significance to ensure the function of chloroplast genetic information and participate in plant growth and development.

Fig. 1. Chloroplast mRNA degradation. (Stern D B, et al., 2010)

Services

The complex RNA metabolism of the chloroplast includes RNA processing, editing, splicing, and regulation of various RNA-binding proteins. When studying chloroplast RNA metabolism, highly purified chloroplasts free of nuclear/cytoplasmic contaminants are required. We have successfully isolated high-quality chloroplasts from mesophyll cells of various plants. Over the years, the team at Lifeasible has accumulated extensive experience in chloroplast metabolomic analysis. Here, our scientists are committed to providing chloroplast RNA metabolism services to analyze RNA transcription, RNA maturation, RNA editing and translation initiation processes. We have a rich database of chloroplast genomes available to help you get all the information about the metabolic composition of RNA in the chloroplasts of land plants. According to the customers' project and requirements, we will choose the right chloroplast RNA metabolism service for you:

· Chloroplast RNA Transcription
We can use a variety of techniques to identify RNA polymerases required for transcription, enabling the use of many different promoters to generate different transcript arrays.

· Chloroplast RNA Editing
We will use comparative genomics strategies to analyze RNA metabolism, identify mutants that are deficient in RNA metabolic events that occur in a species and study how they cooperate with other proteins to perform nucleotide transitions.

· Chloroplast RNA Processing
The pentapeptide repeat (PPR) protein family has attracted much attention as important players in the RNA metabolism of plant organelles. We can help you specifically identify PPR targets, enabling RNA processing to increase transcript species per gene.

Competitive Advantages

• Professional team with rich experience in chloroplast RNA metabolism analysis.
• Studying chloroplast RNA metabolism can fine-tune chloroplast protein sequences that are critical for plant survival.
• Harnessing the vast external resources to tap the coding potential of the chloroplast nuclear genome.
• The experimental process is strictly controlled to ensure the accuracy of the results.
• Fully customized service, providing professional and detailed analysis of results.

Lifeasible can meet the needs of customers on time and on budget through a wide range of chloroplast metabolomics analysis strategies. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

References

1. Stern D B, Goldschmidt-Clermont M, Hanson M R. (2010) Chloroplast RNA metabolism[J]. Annual review of plant biology. 61: 125-155.
2. Wang H, Jin H. (2021) Purification and Analysis of Chloroplast RNAs in Arabidopsis[M]//RNA Abundance Analysis. Humana, New York, NY, 133-141.