Study on the Expression Pattern of Chloroplast Proteins

Chloroplasts are semi-autonomous organelles containing relatively independent genetic systems and capable of self-replication. It is estimated that there are more than 3,000 proteins in the chloroplast. However, the vast majority of them are encoded by nuclear genes, synthesized on cytoplasmic ribosomes, and then transported to chloroplasts, and only a few are encoded by chloroplast genes and translated on chloroplast ribosomes. Thus, chloroplast development involves the coordinated expression of plastid- and nuclear-encoded genes. Analysis of the chloroplast genome sequence showed that the predicted chloroplast genome encodes about 100 proteins. Chloroplast genome-encoded proteins play important roles in photosynthesis, plastid transcription and translation. Studies have found that under different stress conditions, chloroplasts and their encoded proteins will undergo different morphological and physiological changes. In addition, chloroplasts can also be used as bioreactors to efficiently express foreign genes, and even express vaccine and drug proteins.

Fig. 1. Overview of chloroplast gene expression. (Zhang Y, et al., 2020)

Services

Scientists have successfully used two-dimensional electrophoresis combined with mass spectrometry to analyze protein changes in plant leaves and identify multiple differentially expressed proteins. However, this method mainly identifies proteins located in chloroplasts, and studies on proteins encoded by chloroplast genes have paid less attention. Over the years, the team at Lifeasible has extensive experience in studying the expression patterns of proteins encoded by the chloroplast genome. We offer the use of antibody-based proteomic strategies to analyze protein expression in different tissues of plants. In addition, we can help you study the characterization of all proteins in a specific cell or tissue under specific conditions, including changes in protein expression under different stress treatments, under different induction conditions in chloroplast expression systems, or in different chloroplast mutants. Our chloroplast protein expression pattern service process is as follows:

(1) Prepare antibodies specific for proteins encoded by chloroplast genes, including proteins related to photosynthesis, proteins related to RNA replication, and proteins related to NADH dehydrogenase.

(2) Extraction of plant chloroplast proteins and detection and analysis by Western blotting.

(3) Transcriptional analysis of chloroplast-encoded genes, comparing the expression information of the target protein with the transcriptional information.

In conclusion, our service can help you study the expression rules and functions of chloroplast-encoded proteins, which is of great significance for understanding the morphogenesis of chloroplasts, their functions in adversity, and the expression of exogenous proteins.

Competitive Advantages

• Our team has extensive knowledge and research experience in chloroplast gene expression and chloroplast development.
• The polypeptide antigens we designed are specific in the plant chloroplast proteome.
• Antibody-based strategies for plant chloroplast proteomics.
• Comprehensively study the expression of plant chloroplast-encoded proteins.

Lifeasible can meet the needs of customers on time and on budget through a wide range of strategies for analyzing chloroplast proteome. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

References

1. Zhang Y, Zhang A, Li X, et al. (2020) The role of chloroplast gene expression in plant responses to environmental stress[J]. International Journal of Molecular Sciences. 21(17): 6082.
2. Kong M, Wu Y, Wang Z, et al. (2021) A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana[J]. Frontiers in plant science. 12.