Chloroplast Genomics Services

In recent years, scientists have successfully carried out chloroplast genomics research due to the application of modern molecular biology techniques such as recombinant DNA and genetic engineering techniques, as well as the establishment of rapid DNA sequence analysis methods. As an expert in the field of chloroplast engineering, Lifeasible is committed to the study of chloroplast genomic information and provides comprehensive chloroplast genomics services. Our team can use the most advanced technology to study the structure, function and expression of chloroplast genes, and determine the full sequence of chloroplast DNA in a variety of plants.

Introduction

As an important part of plant organelles and the organ of photosynthesis, chloroplasts became the source of energy to advance the evolution of early life. In recent years, with the continuous development of large-scale sequencing technology in molecular biology, the research on the chloroplast genome has become increasingly in-depth. The chloroplast genome database has grown rapidly since the complete sequence of the tobacco and buckwheat chloroplast genomes was first obtained in 1986. The study found that the information of chloroplast genome structure and sequence plays an important role in revealing the origin of species, evolutionary evolution and the relationship between different species. In addition, compared with nuclear transformation, chloroplast transformation technology has obvious advantages in genetic improvement, production of biological agents, etc. However, a prerequisite for chloroplast transformation is a detailed and reliable analysis of the chloroplast genome structure and sequence. Scientists have successfully determined the complete sequence of chloroplast DNA from several higher plants such as tobacco, rice, and maize, which has greatly promoted the identification and understanding of the structure of the chloroplast genome. In conclusion, a comprehensive understanding of the chloroplast genome and its role in biological evolution is of great significance for in-depth research and full play of chloroplast functions in the future.

Fig. 1. Nuclear and chloroplast genome engineering of a productive non-model alga Desmodesmus armatus. (Douchi D, et al., 2021)

Our Services

As the center of photosynthesis, the study of the chloroplast genome is of great significance for revealing the mechanism and metabolic regulation of plant photosynthesis. At the same time, the in-depth study of the chloroplast genome will help to understand the mutual regulation between the nuclear genome and the chloroplast genome. By comparing and repeating analysis of genomes between different species, we will provide you with a wealth of information, including gene composition, gene order, sequence similarity, repeat sequences, etc. Our chloroplast genomics services include, but are not limited to:

• Chloroplast DNA Extraction
• Chloroplast DNA Sequencing Services
• Diversity Analysis of Chloroplast Genome Sequences

Methods for Chloroplast Genome Research

Sequences of chloroplast genomes are growing rapidly, and currently only a small fraction of them are in the database. We employed a variety of chloroplast genome research methods to provide chloroplast genome information for various crops and identify suitable recombination integration sites in the chloroplast genome.

• Enzymatic Digestion
  Understanding the changes in chloroplast genome sequences of different species by comparing the size of the digested fragments.
• Structural Recombination
  Molecular hybridization and PCR were used to compare a large number of chloroplast genomes near recombination sites, and to detect structural changes such as loss of gene, intron inversion, expansion and contraction of IR region, etc.
• Sequencing
  Based on high-throughput sequencing technology, the chloroplast genome sequences of various plants are determined, so as to provide a large amount of genomic information from the comparison between different genomes at the base level.

Lifeasible provides you with professionally and economically oriented chloroplast engineering service projects. Each project will be individually designed to meet our customers’ requirements and described in a detailed project report. Our customers have direct access to our experts and provide immediate feedback on any online inquiries. If you are interested in our services, please do not hesitate to contact us.

References

1. Dobrogojski J, Adamiec M, Luciński R. (2020) The chloroplast genome: a review[J]. Acta Physiologiae Plantarum. 42(6): 1-13.
2. Douchi D, Mosey M, Astling D P, et al. (2021) Nuclear and chloroplast genome engineering of a productive non-model alga Desmodesmus armatus: Insights into unusual and selective acquisition mechanisms for foreign DNA[J]. Algal Research. 53: 102152.