Study on the Interaction Between Chloroplast Proteins

In organisms, proteins do not perform functions alone, but perform various biological functions by interacting with other proteins, nucleic acids or small molecular compounds. Many small molecules can interact with protein molecules. In the chloroplast, protein-protein interactions play important roles in many processes, from the formation of the protein import machinery, to the division machinery, to the formation of the photosynthetic complex. Studies have found that the outcomes of interactions between chloroplast proteins vary widely. Researchers have successfully used a variety of techniques to identify and characterize interactions between chloroplast proteins, including co-immunoprecipitation, yeast hybridization systems, and protein chip arrays. In addition, various computational methods have been developed to predict interactions between proteins.

Fig. 1. The photosynthetic complexes in the chloroplast protein interaction network. (Yu Q B, et al., 2008)

Services

Protein interactions are very important for cell and organelle function. Therefore, scientists need to identify and characterize protein interactions in plants to understand biological processes in the chloroplast. Over the years, the team at Lifeasible has extensive experience in studying chloroplast protein-protein interaction analysis. Our scientists used bimolecular fluorescence complementation and Förster resonance energy transfer to detect protein-protein interactions in intact Arabidopsis living chloroplasts. In addition, we have successfully constructed an Arabidopsis chloroplast protein interaction network to identify and annotate previously uncharacterized proteins. This network is widely used by customers around the world to search for high-confidence protein interaction data, providing a useful resource for further studies of chloroplast protein function and a comprehensive understanding of photosynthetic mechanisms. The basic process of the research service of chloroplast protein interaction is as follows:

(1) Construction of co-transformation and co-expression of fusion proteins in leaf cells.

(2) Visualization and analysis of protein-protein interactions by FRET and BiFC experiments.

(3) Construction of Arabidopsis chloroplast protein interaction network: multiple computational methods were used to detect protein interactions, including phylogenetic analysis, intergene analysis, co-expression, biological processes, enriched domain pairs, and gene fusions.

(4) Functional annotation of previously uncharacterized proteins.

(5) Validation of protein interactions by yeast two-hybrid experiments.

Competitive Advantages

• Helping you comprehensively characterize interactions between chloroplast proteins in plant cells.
• Due to the small size of Arabidopsis thaliana is ideal for microscopy and facilitates live-cell imaging.
• It enables the observation and analysis of protein-protein interactions in the correct cellular environment under physiological conditions.
• The correct subcellular localization of protein interactions can be visualized.
• Multiple computational methods are used to detect protein interactions with fast and reliable results.

Lifeasible can meet the needs of customers on time and on budget through a wide range of strategies for analyzing chloroplast proteome. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

References

1. Yu Q B, Li G, Wang G, et al. (2008) Construction of a chloroplast protein interaction network and functional mining of photosynthetic proteins in Arabidopsis thaliana[J]. Cell research. 18(10): 1007-1019.
2. Maple J, Møller S G. (2011) Studying Interactions Between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation and Förster Resonance Energy Transfer[M]//Chloroplast Research in Arabidopsis. Humana Press, Totowa, NJ. 51-65.