Chloroplast Lipidomics Services

In many eukaryotes and prokaryotes, cell membranes are mainly composed of phospholipids. However, in the chloroplast, an important organelle of the plant, its photosynthetic tissue possesses unique membrane lipid components that support photosynthetic activity by maintaining the thylakoid membrane structure or assembling into the photosynthetic machinery. Chloroplast lipidomics aims to reveal the physiological functions and regulatory mechanisms of chloroplast lipid synthesis. As an expert in the field of chloroplast engineering, Lifeasible is committed to chloroplast lipidomic research, providing reliable lipidomic analysis services for chloroplasts of various plants through biotechnology.

Introduction

Chloroplasts perform oxygenated photosynthesis on the thylakoid membrane. The biogenesis of thylakoid membranes requires the synergistic synthesis and assembly of proteins, pigments, and many photosynthetic cofactors with membrane glycerolipids. The chloroplast membrane is composed of four unique lipids, including monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulfoquinolinyldiacylglycerol (SQDG), and phosphatidylglycerol (PG). Since these lipids constitute a large portion of photosynthetic membranes and have specific roles in the biogenesis and maintenance of thylakoid membranes, as well as in photosynthesis, they play a key role in maintaining chloroplast function. With the development of lipidomics, most of the genes encoding chloroplast lipid synthases have been found, and important progress has been made in revealing the physiological functions and regulatory mechanisms of chloroplast lipid synthesis. Scientists wanted to understand the role of lipids in chloroplast function by exploring the photosynthetic mechanisms involved in lipid biosynthesis during chloroplast biogenesis.

Fig. 1. Glycerolipid synthesis and transport in chloroplasts. (Hölzl G, et al., 2019)

Our Services

• Identification of Lipid Composition and Structure in Chloroplasts
• Analysis of Chloroplast Lipid Biosynthesis
• Analysis of the Function of Chloroplast Lipids

In addition, our team used advanced genetics and biochemical techniques to identify genes involved in chloroplast lipid biosynthesis and to analyze the regulation of these genes in chloroplasts under various growth conditions. We have successfully established a well-established workflow for chloroplast lipidomics:

(1) Clarify customer project requirements.
(2) Lipidomics analysis: including sampling and sample preparation, separation and identification, and data processing.
(3) Interpretation of the results: the metabolic pathways related to lipid compounds were obtained, and the effect of chloroplast lipid biosynthesis pathway on chloroplast function was analyzed.

Methods for Chloroplast Lipidomics Research

Our dedicated lipidomics team has extensive experience in chloroplast lipidomics analysis. In addition, the lipid compounds in chloroplasts are various and the biological sample matrix is complex, so we provide customers with the most reliable lipidomics analysis with the help of advanced separation technology and detection methods:

• Thin layer chromatography (TLC) method.
• Gas chromatography (GC) method.
• Capillary electrophoresis (CE) method.
• Supercritical fluid chromatography (SFC).
• High performance liquid chromatography (HPLC) method.
• Various mass spectrometry (MS) techniques.
• Chromatography-Mass spectrometry.
• Mass spectrometry combined with nuclear magnetic resonance (NMR), infrared spectroscopy and Raman spectroscopy.

Lifeasible provides you with professionally and economically oriented chloroplast engineering service projects. Each project will be individually designed to meet our customers’ requirements and described in a detailed project report. Our customers have direct access to our experts and provide immediate feedback on any online inquiries. If you are interested in our services, please do not hesitate to contact us.

References

1. Hölzl G, Dörmann P. (2019) Chloroplast lipids and their biosynthesis[J]. Annual Review of Plant Biology. 70: 51-81.
2. Kobayashi K, Wada H. (2016) Role of lipids in chloroplast biogenesis[J]. Lipids in plant and algae development. 103-125.