Diversity Analysis of Chloroplast Genome Sequences

The availability of more than 800 sequenced chloroplast genomes from a variety of terrestrial plants has successfully deepened our research on chloroplast biology, intracellular gene transfer, conservation, diversity, and by engineering chloroplast transgenics. Lifeasible's expertise in chloroplast engineering enables us to provide specialized chloroplast genome sequence diversity analysis services, allowing the chloroplast protein coding regions and conserved sequence genomes to be fully analyzed. If you have any special requirements about our diversity analysis of chloroplast genome sequences service, please feel free to contact us.

Diversity of Chloroplast Genome Sequences

The chloroplast genome is maternally inherited and has a coherent structure consisting of two inverted repeat regions (IR), a large (LSC) and a small (SSC) single-copy region. Two strategies are currently used, involving the use of the complete chloroplast genome to distinguish plant species, or the search for mutational hotspots and the use of comparative plastid analysis to find loci with appropriate species-level differences. Analysis of multiple complete chloroplast genome sequences provides important support for the execution of both strategies. In addition, in-depth chloroplast genome sequence analysis is of great value for studies of plant population genetics, phylogenetic reconstruction, species identification, and genome evolution.

Fig. 1. Chloroplast genome structure and gene expression across tracheophytes. (Singh N V, et al., 2021)

Services

Chloroplast genomes provide very rich information in species identification, phylogenetic significance and population genetic analysis, so it is very necessary to conduct chloroplast genome analysis. Lifeasible has abundant chloroplast genome resources and is committed to providing professional chloroplast genome sequence diversity analysis services to determine phylogenetic relationships, pattern evolution, and interspecific genetic diversity. Based on the complete chloroplast genome sequence, we provide the following chloroplast genome sequence analysis services:

• Comparative Analysis of Chloroplast Genomes
  We can help you compare the chloroplast genomes of various plants with databases in Genome Resources to determine their chloroplast genome characteristics. And multiple alignments of chloroplast genomes were performed to study the level of genomic divergence.
• Analysis of Variation In Chloroplast DNA Sequences
  We identified and designed highly suitable polymorphic chloroplast loci as unique markers in different groups. To facilitate the identification of closely related species, we sought to identify potential molecular markers of the hypervariable regions of the chloroplast genome for species identification and evolutionary studies.
• Repeat Sequence Analysis of Chloroplast Genomes
  We analyzed repetitive sequences (long repetitive sequences and simple sequence repeats) of the chloroplast genome to understand sequence differences and the evolutionary history of plants.
• Analysis of Chloroplast Genome Phylogenetic
  We can use large-scale molecular data at the genome level to study phylogenetic relationships between organisms and use evolutionary relationships in turn to study the evolutionary mechanisms of genomes.

Competitive Advantages

• Compared with mitochondrial and nuclear genomes, chloroplast genome analysis has many advantages, such as small size, low nucleotide substitution rate, uniparental inheritance, and highly conserved genome structure.
• The chloroplast genome is highly conserved, evolves slowly, and does not undergo recombination.
• Chloroplast DNA (cpDNA) is widely used for species identification and evolutionary analysis.
• The complete chloroplast genome sequence provides important support.
• Professional team, high-end data analysis platform.

References

1. Daniell H, Lin C S, Yu M, et al. (2016) Chloroplast genomes: diversity, evolution, and applications in genetic engineering[J]. Genome biology. 17(1): 1-29.
2. Li Q, Su N, Zhang L, et al. (2020) Chloroplast genomes elucidate diversity, phylogeny, and taxonomy of Pulsatilla (Ranunculaceae)[J]. Scientific reports. 10(1): 1-12.