Isolation and Preparation of Chloroplasts

The isolation and preparation of active isolated chloroplasts is the premise of studying the structure and function of chloroplasts, the principle of photosynthesis and the mechanism of genetic control. As an expert in the field of chloroplast engineering, Lifeasible is committed to studying the structure and function of chloroplast, and providing comprehensive chloroplast isolation and preparation services. Our team can use state-of-the-art technology to isolate and purify chloroplasts to obtain chloroplasts, chloroplast inner and outer membranes and chloroplast thylakoid membranes not only from higher plants, but also from healthy mesophyll protoplasts.

Introduction

Chloroplasts are found in the mesophyll cells of leaves, which are organelles in plants with a size of 5-10 microns and have the function of photosynthesis. The chloroplast contains 3 different membranes (outer membrane, inner membrane, thylakoid membrane) and 3 separate lumens (intermembrane space, stroma and thylakoid lumen). According to the structural integrity of the isolated chloroplasts, the chloroplasts can be roughly divided into two categories: one is the chloroplast whose membrane has been broken, called broken chloroplast, which has the functions of photosynthetic electron transfer, photosynthetic oxygen release and photosynthetic phosphorylation. The other type is the intact chloroplast, which has the complete photosynthetic function of assimilating carbon dioxide. The isolation and preparation of active isolated chloroplasts is the premise of studying the structure and function of chloroplasts, the principle of photosynthesis and the mechanism of genetic control. In addition, chloroplast fractions can be further extracted to obtain membrane, matrix or thylakoid proteins as well as chloroplast DNA and RNA.

Fig. 1. Flowchart of protocol for isolation of intact chloroplasts followed by NGS sequencing of chloroplast genome. (Vieira L N, et al., 2014)

Our Services

The chloroplasts isolated and prepared are widely used in biochemical, cellular and molecular analysis to facilitate the study of chloroplast structure and function. Among them, the membrane integrity rate of chloroplasts can reach more than 95%, sometimes even 100%. Our chloroplast isolation and preparation services include but not limited to:

• Isolation of Chloroplasts from Plant Tissue
• Isolation of Chloroplast Inner and Outer Envelope Membranes
• Isolation of Chloroplast Thylakoid Membranes
• Isolation of Chloroplast Thylakoid Lumen

Available Advantages

• Saving time with simple and proven protocols that are easy to follow.
• Isolation of intact chloroplasts can be used to study processes such as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting.
• Isolated chloroplasts can be used for functional studies, metabolic transport, proteomic analysis and genomics.
• Complete experimental records and detailed experimental results, the process is simple and waste is reduced.
• Achieving chloroplast isolation of various plants such as spinach, pea, lettuce, cabbage, chlamydomonas, chlorella.

Lifeasible provides you with professionally and economically oriented chloroplast engineering service projects. Each project will be individually designed to meet our customers’ requirements and described in a detailed project report. Our customers have direct access to our experts and provide immediate feedback on any online inquiries. If you are interested in our services, please do not hesitate to contact us.

Reference

1. Vieira L N, Faoro H, Fraga H P F, et al. (2014) An improved protocol for intact chloroplasts and cpDNA isolation in conifers[J]. PLoS One. 9(1): e84792.