Qualitative and Quantitative Analysis of Chloroplast Metabolites

A major goal of metabolomics is to measure the complete range of primary and secondary metabolites present in or transported from a biological system to better understand the precise biological role of that system at a specific developmental stage or under specific conditions or functional environmental factors. More than 200,000 known plant secondary metabolites have been identified. Metabolism is involved in signaling, physiological regulation, and defense responses when the environment is unfavorable and plant growth is affected. As a major plant organelle, chloroplasts have basic metabolic and biosynthetic functions. Scientists have successfully constructed chloroplast metabolism models to study chloroplast photosynthesis under light and dark conditions. In addition, plastid globules are lipoprotein structures attached to the thylakoid membrane and are involved in chloroplast metabolism and stress responses. Scientists quantitatively evaluate plastid sphere occurrence and morphology through comprehensive studies to infer the precise response of chloroplast metabolism. Chloroplast metabolite profiling and metabolic pathway mapping provide critical information for the search for new trait-enhanced crops.

Fig. 1. Photosynthetic metabolism in the chloroplasts of C3 plants. (Luo R, et al., 2009)

Services

Metabolomics is increasing our knowledge of how complex metabolic networks interact and how they are dynamically modified during stress adaptation and tolerance through the qualitative and quantitative analysis of a large number of metabolites. Over the years, the team at Lifeasible has accumulated extensive experience in chloroplast metabolomic analysis. Given the different physicochemical properties of different metabolites, no single analytical technique is sufficient to cover the entire metabolome of chloroplasts. Our engineers are committed to providing the most comprehensive and professional qualitative and quantitative analysis services for chloroplast metabolites to customers, using chromatographic techniques including mass spectrometry to qualitatively and quantitatively analyze various metabolites involved in important biosynthetic pathways and processes.

In addition, given the dynamic and highly labile nature of most metabolites, we performed the chloroplast isolation process in an inert environment with minimal loss or degradation of metabolites. Lifeasible has successfully established a model of plant chloroplast photosynthesis. Reliable and rapid analysis of various metabolites in chloroplasts by the following procedures:

(1) Isolation of chloroplasts.
(2) Metabolites identified from isolated samples.
(3) The division of major classes of metabolites was estimated by a three-compartmental analysis method.
(4) Spatial analysis of metabolites by GC-MS or LC-MS.
(5) Hierarchical cluster analysis was performed on the most abundant metabolites among the total identified metabolites.

Competitive Advantages

• Professional team with rich experience in chloroplast photosynthesis and metabolism analysis.
• We can provide accurate information on the structure and properties of metabolites, and more accurately reflect the distribution of metabolic processes within cells or tissues.
• Integrated chromatography technology provides faster, more accurate data and requires less material.
• Fully customized service, providing professional and detailed analysis of results.

Lifeasible can meet the needs of customers on time and on budget through a wide range of chloroplast metabolomics analysis strategies. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

References

1. Luo R, Wei H, Ye L, et al. (2009) Photosynthetic metabolism of C3 plants shows highly cooperative regulation under changing environments: a systems biological analysis[J]. Proceedings of the National Academy of Sciences. 106(3): 847-852.
2. Sárvári É, Gellén G, Sági-Kazár M, et al. (2022) Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE[J]. Plant methods. 18(1): 1-19.