Chloroplast Genetic Engineering via Somatic Embryogenesis

Chloroplast genetic engineering has gained widespread popularity due to its many unique advantages. Today, chloroplast genetic engineering is one of the key technologies for crop improvement and an emerging way to produce recombinant proteins in plants. Chloroplast transformation is very efficient in tobacco. Scientists have successfully integrated and expressed more than 40 transgenes stably through the tobacco chloroplast genome, conferring desired agronomic traits or expressing high levels of vaccine antigens and biopharmaceuticals. However, major obstacles to extending this technology to crop plants regenerated by somatic embryogenesis include inadequate tissue culture and regeneration protocols, lack of selectable markers, and inability to express transgenes in non-green plastids. Arabidopsis thaliana as an ideal model system for chloroplast functional genomics. The researchers were pleasantly surprised to find that it is feasible to regenerate Arabidopsis through somatic embryogenesis. Therefore, extending this technique to other important plant species has been a research hotspot.

Fig. 1. Schematic structure and prokaryotic functional features of plastid transformation vectors. (Meyers B, et al., 2010)

Services

Over the years, the team at Lifeasible has accumulated extensive experience in chloroplast genetic engineering and is commmitted to extending chloroplast genetic engineering to crop plants that regenerate through somatic embryogenesis. Here, we successfully achieved efficient soybean, carrot and cotton plastid transformation via somatic embryogenesis using vectors containing homologous native flanking sequences from tobacco or Arabidopsis, species-specific chloroplast-based vectors. We also provide chloroplast transformation through somatic embryogenesis in other crops, including grains, beans, oil crops, cash crops, vegetable crops, fruits, etc.

• Transformation of the Carrot Plastid Genome
• Transformation of the Cotton Plastid Genome
• Transformation of the Soybean Plastid Genome

Advantages of Our Services

• Use of homologous native flanking sequences in tobacco or Arabidopsis improves plastid transformation efficiency.
• Providing plastids with the ability to survive selection in light and darkness at different developmental stages.
• The presence of chloroplast DNA origins of replication in long flanking sequences provides additional templates for transgene integration.
• The transcriptionally active spacer has a highly preferred site where more transgenes are integrated than any other site.
• Paving the way for designing plastid genomes of several major crops
• Plant regeneration is achieved by somatic embryogenesis.

Lifeasible can meet the needs of customers on time and on budget through a wide range of chloroplast genetic engineering strategies. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information.

References

1. Dhingra A, Daniell H. (2006) Chloroplast genetic engineering via organogenesis or somatic embryogenesis[M]//Arabidopsis protocols. Humana Press. 245-262.
2. Meyers B, Zaltsman A, Lacroix B, et al. (2010) Nuclear and plastid genetic engineering of plants: comparison of opportunities and challenges[J]. Biotechnology advances. 28(6): 747-756.