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- Chloroplast Engineering of Drought Tolerance
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Through plastid genome engineering, we have achieved agronomic transformation of many important crops, such as herbicide resistance, drought tolerance, salt tolerance, insect resistance, pathogen resistance, nitrogen fixation, nutrition, and cytoplasmic male sterility. Here, Lifeasible is committed to providing crops with reliable and economical chloroplast genetic engineering solutions to develop crops with enhanced drought resistance.
Drought is one of the environmental pressures that affects the productivity of most field crops and poses a major threat to food security. Therefore, humans must develop agricultural strategies to deal with water scarcity, grow plants in short climatic intervals when water resources are available, and select plants that are relatively tolerant of water scarcity. Plants utilize a variety of strategies to cope with drought stress and adapt to drought through diverse signaling cascades and osmotically regulated morphological and physiological changes. New crop varieties with higher drought resistance have been developed by conventional breeding methods. Chloroplasts are specialized photosynthetic organelles that are vulnerable to damage by many environmental stresses, and scientists have successfully genetically engineered crop chloroplasts to respond to drought stress.
Fig. 1. Drought tolerance genes that have been discovered or tested in model species and translated successfully into crop species. (Martignago D, et al., 2020)
Plastid transformation technology retains great potential for genetic studies of plant responses to water stress. We have successfully applied chloroplast transformation technology to drought tolerance of crops. Lifeasible is committed to integrating multiple drought-tolerant genes into the chloroplast genome for engineering studies of drought-tolerant crops.
Plant genetic engineering provides the possibility to control the plant genome towards more specific and grand goals, study gene structure and function, and determine the relationship between gene expression and causal factors. Our engineers use biotechnology to improve the most promising phenotypic drought trait for drought resistance in rice. In addition, we identify and manipulate novel genes that may confer drought resistance through current genome editing technologies. Here, we currently offer the following solutions to impart drought resistance to crops.
(1) Early Flowering and Drought Escape Mediated Drought Response
We overexpressed the OsFTL10 gene in transgenic rice plants, resulting in earlier flowering and improved drought tolerance.
(2) Leaf Traits Mediated Drought Response
We improved the photosynthetic activity and drought performance of dicots by manipulating ethylene biosynthesis genes such as HaHB4.
(3) Stomatal Mediated Drought Response
We overexpressed the AtNF-YB1 gene and the ABA receptor pyrabactin resistance 1-like 1 (pyl1), pyl4 and pyl6 in transgenic crops to improve drought tolerance.
(4) Carbon Allocation Mediated Drought Response
We overexpressed OsNACs, OsERF71, HVA1, and DRO1 genes in transgenic crops to improve drought and high salt resistance by expanding root diameter.
Lifeasible's goal is to provide customers around the world with fully customized chloroplast engineered solutions for drought tolerance. Please contact us to discuss further details to ensure your next success.
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