Chloroplast DNA Sequencing Services

Chloroplast genome sequencing is generally a high-throughput sequencing of plant chloroplasts. Through high-depth sequencing and bioinformatics analysis, relevant information such as the sequence, coding genes, and genetic evolution of the chloroplast genome was obtained. Lifeasible's expertise in chloroplast engineering has allowed us to sequence the chloroplast genomes of a wide variety of plants using advanced sequencing technologies, which can be used to support sound phylogenetic reconstruction and chloroplast genetic transformation engineering. If you have any special requirements about our chloroplast DNA sequencing service, please feel free to contact us.

Chloroplast DNA (cpDNA) Sequencing

The chloroplast is one of the most indispensable and unique organelles in green plants. As semi-autonomous genetic organelles, chloroplasts have their own genetic material, the chloroplast genome (cpDNA). cpDNA contains a wealth of information on speciation, making it a rich resource for tracking population-level processes and evolutionary divergence. In addition, the structural complexity of the chloroplast genome is much simpler than that of the nuclear genome and often has a highly conserved organization and gene content across species. The chloroplast genomes from many terrestrial plants and algae have been sequenced. However, challenges remain in rapidly and cost-effectively sequencing or resequencing the chloroplast genome. Traditional sequencing begins with the construction of plastid or other genomic libraries, a complex and time-consuming process. Scientists have been working hard to find a simple and fast chloroplast gene sequencing method, which has important implications for species identification, phylogenetic significance, and population genetic analysis.

Fig. 1. Sequencing and assembly strategies. (Edwards D, et al., 2010)

Services

With continued innovation in next-generation sequencing (NGS) technologies and the reduction in sequencing costs, whole chloroplast genome sequencing can now be performed very rapidly and in a cost-effective manner. Lifeasible has successfully built a variety of advanced sequencing technology platforms to sequence the chloroplast genomes of various plants. We provide sequencing of isolated cpDNA, PCR amplification and total genomic DNA to obtain chloroplast genome sequence, and choose the appropriate isolation and sequencing service for you according to your project:

• Sequencing Service Based on Isolated cpDNA
  We isolated intact chloroplasts from mesophyll cells and extracted cpDNA from chloroplasts for sequencing.
• Sequencing Service Based on PCR-Amplified cpDNA
  We obtain sequences by sequencing long cpDNA fragments amplified using PCR.
• Sequencing Service Based on Whole Genome cpDNA
  We sequence the total DNA (including nuclear genomic DNA, cpDNA), and then screen out the cpDNA sequence for splicing and assembly to obtain a complete chloroplast genome.

We are committed to providing you with cost-effective cpDNA sequencing services, from chloroplast DNA preparation, chloroplast enrichment, chloroplast DNA sequencing to analysis of the entire chloroplast genome from total DNA samples. Our services will support rapid sequencing of chloroplast DNA genomes faster and more cost-effectively. Our advanced technologies include:

• Based on high-throughput sequencing technology.
• Based on next-generation sequencing (NGS) method.
• Based on single-molecule real-time (SMRT) sequencing.
• Based on Sanger sequencing method.
• Based on Illumina sequencing method.

Competitive Advantages

• We can use the appropriate sequencing solution for you to ensure the accuracy of the results according to different requirements.
• Our team has completed chloroplast DNA sequencing of multiple species and has extensive sequencing experience.
• Advanced technology platforms support efficient, cost-effective sequencing.
• Complete and detailed data analysis and recording, more data mining can be done according to your requirements.

References

1. Edwards D, Batley J. (2010) Plant genome sequencing: applications for crop improvement[J]. Plant biotechnology journal. 8(1): 2-9.
2. Ni Z X, Ye Y J, Xu M, et al. (2020) Comparison among three methods for obtaining chloroplast genome sequences from the conifer Pinus massoniana[J]. Genomics. 112(3): 2459-2466.