Chloroplast Genetic Engineering

With the deepening of chloroplast genome research, people have gradually realized chloroplast transformation has become possible to direct exogenous genes into the chloroplast genome and realize their expression. As an innovator in chloroplast engineering, Lifeasible is committed to the research of chloroplast transformation and provides comprehensive chloroplast genetic engineering services. Our team can use gene recombination or gene splicing technology to integrate genes from different sources into recombinant DNA molecules and express them in living cells to change the original genetic characteristics of organisms, obtain new varieties and produce new products.

Introduction

The chloroplast has a unique genome that replicates itself, and is an important site for photosynthesis. But after millions of years of evolution, most of the chloroplast's genes are under nuclear control. The three functional genes of photosynthesis, expression regulation and biosynthesis are mainly retained in the chloroplast genome. With the rapid development of molecular biology, chloroplast genomes of various plants have been obtained by various sequencing methods. Chloroplast genes have the characteristics of small molecular weight, simple structure and easy inheritance. In addition, the chloroplast gene itself has a huge copy number, so it can express the target gene more efficiently than the traditional nuclear inheritance. Scientists have successfully used chloroplast genome transformation techniques to improve crop traits and to develop biomaterials and therapeutic proteins. At present, chloroplast genetic engineering has attracted much attention because of its unique advantages, and has become a new research hotspot in plant genetic engineering.

Fig. 1. Chloroplast engineering: basic layout and its applications. (Verma A, 2019)

Our Services

Using plant-specific chloroplast vectors such as soybean, carrot, and cotton, our engineers inserted, integrated, and expressed multiple genes from different sources through somatic embryogenesis, and successfully achieved efficient plastid transformation. We can use chloroplast genome transformation technology to help you carry out basic research on plant biosynthesis, chloroplast gene structure, genome evolution, gene transcription, gene translation, RNA editing, etc. Our chloroplast genetic engineering services include, but are not limited to:

• Construction of Chloroplast Transformation System
• Antibiotic-Free Chloroplast Genetic Engineering
• Chloroplast Genetic Engineering via Somatic Embryogenesis
• Engineering Chloroplast Genomes to Resist Pathogens
• Optimization of Foreign Gene Expression in Chloroplast

Advantages of Chloroplast Genetic Engineering

• High-efficiency expression: exogenous genes from prokaryotes can be highly expressed in chloroplasts without modification and modification.
• Good safety: after the foreign gene is integrated into the chloroplast genome, it will not spread, so there is no "gene escape" problem.
• Successfully achieved site-directed integration of exogenous genes into chloroplasts in tobacco, Arabidopsis and potato.
• The chloroplast genome is small, simple in structure, and does not bind to histones, making genetic manipulation easy.
• A mature chloroplast gene transformation platform, providing comprehensive, reliable and professional chloroplast engineering services.

Lifeasible provides you with professionally and economically oriented chloroplast engineering service projects. Each project will be individually designed to meet our customers' requirements and described in a detailed project report. Our customers have direct access to our experts and provide immediate feedback on any online inquiries. If you are interested in our services, please do not hesitate to contact us.

References

1. Verma A. (2019) 9 Chloroplast genetic engineering: Concept and industrial applications[J]. Industrial Biotechnology: Plant Systems, Resources and Products. 173.
2. Grevich J J, Daniell H. (2005) Chloroplast genetic engineering: recent advances and future perspectives[J]. Critical Reviews in Plant Sciences. 24(2): 83-107.