Production of Chloroplast-Derived Vaccine Antigens

Unlike bacterial and mammalian expression systems, plants are ideal for producing clean, safe, contaminant-free vaccine antigens. Lifeasible has successfully developed a variety of reliable and economical solutions for chloroplast engineering in biopharmaceuticals. Our engineers focus on developing multiple vaccines against bacterial pathogens, viral pathogens and animal pathogens using chloroplast transformation methods.

Current Status of Vaccine Antigen Production

With the development of recombinant technology and immunology, new recombinant vaccines have been widely used in modern medicine. The recombinant vaccines is of great significance for preventing global infectious disease outbreaks and epidemics. Researchers have long used bacteria, yeast, or cultured animal or human cell lines to produce recombinant vaccines. However, these methods have certain drawbacks, such as high cost and limited availability of transformants. Interestingly, chloroplasts transformation can produce recombinant vaccines in an economical and stable manner with improved benefits. In addition, the development of chloroplast engineering technology to produce vaccines from edible plant parts such as tomato fruit and carrot root will enable oral vaccine antigens.

Fig. 1. An outline for the process of oral delivery of plant-derived vaccine antigens. (Chan H T, et al., 2015)

Our Solutions

• Accumulate large amounts of recombinant protein in edible parts of plants, such as carrots, tomatoes.
• Antibiotic resistance genes of selected transformants were removed.
• Antigens are delivered by injection of conjugated adjuvants.
• Oral administration using antigens bio-encapsulated in plant cells.

Our engineers produce a variety of functional oral vaccines against bacterial, viral, and animal pathogens by engineering the chloroplast genomes of algae or higher plants. At present, we can provide solutions for chloroplast engineering in vaccine antigens production, including but not limited to:

• Production of Chloroplast-Derived Cholera Vaccines
• Production of Chloroplast-Derived Anthrax Vaccines
• Production of Chloroplast-Derived Plague Vaccines
• Production of Chloroplast-Derived Canine Parvovirus Vaccines

Attractive Advantages of Our Solutions

• Does not require expensive fermentation systems and is produced in a hydroponics greenhouse.Implantation allows for a rapid increase in the supply of large numbers of subunit vaccines, especially useful against diseases affecting large numbers of people.
• Post-translational modifications can be performed in chloroplast transformation, but not in microbial cell lines.
• Plant chloroplasts are free of human pathogens, reducing the risk of contamination.
• Fusion of antigens to transmucosal carriers can significantly improve the efficiency of their delivery to the immune system.
• Using cold chain-free vaccine delivery methods.

Fig. 2. The flow of of chloroplast-derived vaccine antigens production.

Lifeasible's goal is to provide customers around the world with fully customized chloroplast engineered solutions for vaccine antigens. It should be noted that we only provide solutions for preclinical vaccine development, but do not produce vaccines that can be used directly, and do not conduct clinical trials. Please contact us to discuss further details to ensure your next success.

Reference

1. Chan H T, Daniell H. (2015) Plant-made oral vaccines against human infectious diseases—are we there yet?[J]. Plant biotechnology journal. 13(8): 1056-1070.