Repeat Sequence Analysis of Chloroplast Genomes

Repeated sequences are important genetic markers and are closely related to the origin and evolution of species. Studies have shown that oligonucleotide repeats are the key to generating indels, inversions and substitutions. Long repetitive sequences (30-49 bp) play an important role in chloroplast genome evolution, genome rearrangement and phylogeny studies. Simple sequence repeats (SSRs) are formed by repeated sequences of 1-6 bp in length and are widely used in breeding studies, population genetics, and genetic linkage mapping. SSRs often have natural polymorphisms, and chloroplast SSR is the key content of chloroplast genome repeat sequence analysis. Inverted repeats (IRs) are common higher-order DNA secondary structures in plants and are involved in important biological processes such as gene silencing, DNA replication, and genome stability. Researchers have successfully made new progress in big data analysis and database construction of inverted repeats in plant chloroplast genomes. At present, the content of bioinformatic analysis of chloroplast genome is still relatively large.

Fig. 1. Analysis of repeat sequences in eight chloroplast genomes: (A) frequency of repeat types; and (B) frequency of the repeats by length ≥30 bp. (Gu C, et al., 2018)

Services

Articles on chloroplast genomes often do repeat analysis, which involves the use of a variety of online tools to analyze repeat sequences in the chloroplast genome of plants. Over the years, the team at Lifeasible has extensive experience in chloroplast genome repeat analysis. We are committed to providing global customers with complete chloroplast genome repeat analysis services, including long repeat sequences and simple repeat sequences. Our chloroplast genome repeat analysis service can provide valuable information for your chloroplast research, such as sequence differences, the evolutionary history of plants, etc.

• Chloroplast long repeat analysis service:
  Our scientists analyzed and compared forward repeats, complement repeats, inverted repeats, and palindromic repeats in plant chloroplast genomes using a variety of online tools. The results of our analysis inform the development of genomic markers for population and phylogenetic studies.
• Chloroplast simple repeat analysis service:
  Our scientists use a variety of online tools to analyze microsatellite repeats in plant chloroplast genomes, including mono-, di-, tri-, tetra-, penta-, and hexanucleosides nucleotide repeats. The results of our analysis inform phylogenetic reconstruction and genetic diversity.

Competitive Advantages

• Experience in bioinformatics analysis.
• Using a variety of online tools, simple and fast.
• Specialized chloroplast genome assembly analysis tool software.
• A multi-strategy approach was used for gene prediction.
• Complete and detailed results analysis report.

Lifeasible can meet the needs of customers on time and on budget through a wide range of strategies for analyzing chloroplast genome sequences. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

Reference

1. Gu C, Tembrock L R, Zheng S, et al. (2018) The complete chloroplast genome of Catha edulis: a comparative analysis of genome features with related species[J]. International Journal of Molecular Sciences. 19(2): 525.