Sequencing Service Based on Isolated cpDNA

Scientists have recently used several efficient and improved protocols to obtain high-quality chloroplast DNA, facilitating robust sequencing and further functional genomics studies. Researchers have long ago successfully sequenced whole chloroplast genomes of rice, Arabidopsis and maize using the Sanger technique, providing a fundamental understanding of genome structure and function. Now, NGS technology offers hope for further development of chloroplast genome research. Many researchers are beginning to explore chloroplast genome sequences. This high-throughput, high-resolution analysis has provided complete chloroplast genome sequences for more than 2,000 plant species, greatly facilitating chloroplast genome-focused population genetics and evolutionary studies. The main difficulty in obtaining chloroplast genome sequences is the elimination of nuclear and mitochondrial genomic DNA interference. At present, the commonly used methods for obtaining chloroplast genome sequences mainly include sequencing isolated cpDNA, sequencing PCR amplification and sequencing total genomic DNA. Among them, sequencing isolated cpDNA is the most direct method. Scientists have successfully used this method to sequence the chloroplast genomes of 13 species of Metasequoia and Camellia.

Fig. 1. Flowering plant cp genome sequences. (Du F K, et al., 2015)

Services

We have successfully isolated intact chloroplasts from the mesophyll cells of various plants. Over the years, the team at Lifeasible has accumulated extensive experience in sequencing chloroplast genes. Here, our scientists are dedicated to providing sequencing service based on isolated cpDNA, using Illumina next-generation sequencing technology to sequence complete chloroplast genomes extracted from a variety of plants and obtain high-quality genome sequences, coding genes, genetic evolution, etc. In addition, we compared the chloroplast genome sequences of genotypes of different species with our established cpDNA library to mine more information and confirm the reliability of our sequence data. The flow of sequencing services based on isolated cpDNA is as follows:

(1) Purify chloroplasts and isolate cpDNA to obtain high-quality cpDNA.

(2) Sequence cpDNA by Illumina next-generation sequencing technology, and annotate the sequenced genome.

(3) Sequence alignment and phylogenetic analysis to mine more information, including genome feature analysis, comparative genome analysis, feature primer development, evolutionary analysis and species identification, personalized analysis, etc.

(4) Providing a complete data analysis report.

Competitive Advantages

• Sequence chloroplast genomes in a more cost-effective, time-saving manner and dramatically increase sequence throughput.
• Highly specific, with less interference from nuclear and mitochondrial genomic DNA.
• Not suitable for species high in secondary metabolites and fiber. Widely used in low fiber plants from which chloroplasts are easily isolated.
• There are strict experimental procedures from DNA extraction and quality inspection, to library construction and on-machine sequencing, thus ensuring high data accuracy.
• In addition to providing standard bioinformatics services, also provide personalized bioinformatics services.

Lifeasible can meet the needs of customers on time and on budget through a wide range of chloroplast DNA sequencing  strategies. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

Reference

1. Du F K, Lang T, Lu S, et al. (2015) An improved method for chloroplast genome sequencing in non-model forest tree species[J]. Tree genetics & genomes. 11(6): 1-14.