Chloroplast Proteome Analysis Services

The development of new technologies and the increasing availability of plant genome sequence data have driven the development of the entire chloroplast proteome. Chloroplast proteomics has provided many new insights into chloroplast biogenesis, adaptation and function. Lifeasible's expertise in chloroplast engineering enables us to provide comprehensive chloroplast proteome analysis services including expressed proteins, mutant protein studies, RNA expression, protein-protein interactions, post-translational modifications, protein structure, etc. If you have any special requirements about our chloroplast proteome analysis services, please feel free to contact us.

Chloroplast Proteomics

Proteomics is a field of research that uses two-dimensional electrophoresis separation and mass spectrometry techniques and bioinformatics to elucidate the structural and functional patterns of proteins in organisms. In recent years, proteomic research based on two-dimensional electrophoresis technology has gone deep into the subcellular level, that is, to study the proteome expressed in an organelle. Since chloroplasts are unique organelles of plants and are easier to separate than other organelles, they are the most intensively studied organelles in plants. Rapid improvements in two-dimensional electrophoresis (2-DE) and mass spectrometry, combined with the mining of sequence data from various plant species, have enabled the rapid determination of chloroplast proteins and their expression levels and post-translational modifications. In addition, using the chloroplast proteome as an entry point to conduct subcellular proteomics research can not only reduce the complexity of plant whole cell protein analysis, but also ensure the reliability of the research results. The development of chloroplast proteomics has provided important value for understanding chloroplast biogenesis, environmental adaptation, signal transduction and metabolic pathways.

Fig. 1. Integrated physiological and chloroplast proteome analysis of wheat seedling leaves under salt and osmotic stresses. (Zhu D, et al., 2021)

Services

Chloroplast proteome is an important technical means to obtain chloroplast protein information and determine its function. Lifeasible is committed to providing high-quality chloroplast proteome analysis services to customers around the world. Our scientists systematically identify proteins in organelles (chloroplast membrane, soluble chloroplast matrix proteins, and thylakoid proteins) by proteomics, providing you with the latest information on subcellular protein localization. Additionally, we can help you identify putative, putative or unknown chloroplast proteins. We will also combine proteomic data with data obtained from transcriptional profiling and metabolomics to analyze the level of regulation of chloroplast gene expression. In conclusion, our services can help you gain a comprehensive understanding of the composition, characteristics of chloroplast proteins and their roles in the chloroplast metabolic network. Our chloroplast proteome analysis services include but are not limited to:

• Study on the Expression Pattern of Chloroplast Proteins
• Study on the Functional Model of Chloroplast Proteins
• Study on the Interaction Between Chloroplast Proteins
• Analysis of Chloroplast Outer and Inner Membrane Proteins
• Proteomic Analysis of Chloroplast Biogenesis Mutants
• Systematic Identification and Targeted Analysis of Intraluminal and Peripheral Thylakoid Proteins
• Subcellular Localization and Prediction of Chloroplast Proteins
• Post-translational Modifications of Chloroplast Proteins

Competitive Advantages

• Our professional team has extensive experience in proteomic analysis.
• Providing a complete and highly customized chloroplast proteomic analysis service.
• Combining careful analysis of changes in chloroplast RNA, protein and metabolite levels.
• Fast, cost-effective results analysis. 24h after-sales consulting service.

References

1. Van Wijk K J. (2000) Proteomics of the chloroplast: experimentation and prediction[J]. Trends in plant science. 5(10): 420-425.
2. Zhu D, Luo F, Zou R, et al. (2021) Integrated physiological and chloroplast proteome analysis of wheat seedling leaves under salt and osmotic stresses[J]. Journal of Proteomics. 234: 104097.