Chloroplast Proteomics Services

As an important organelle in photosynthesis, chloroplast proteomics has become a research hotspot, involving areas including chloroplast total proteomics, subcellular proteomics, differential proteomics and protein functions. As an expert in the field of chloroplast engineering, Lifeasible is committed to the research of chloroplast proteomics, using advanced technology to provide reliable proteomic analysis services for chloroplasts of various plants. Our team has successfully applied various separation and mass spectrometry techniques to identify protein species and membrane composition in chloroplasts.

Introduction

Proteomics is a field of research that applies two-dimensional electrophoresis separation and mass spectrometry techniques and bioinformatics to elucidate the structural and functional patterns of proteins in organisms. In recent years, with the advancement of research techniques and means, the research on chloroplast proteome analysis of higher plants has made great progress. Since chloroplasts arise from cyanobacteria through endosymbiosis and are semiautonomous organelles, chloroplasts serve as a paradigm for organelle proteomics. Researchers have successfully used mass spectrometry to identify 916 Arabidopsis chloroplast proteins encoded by nuclear genes with high confidence, 86% of which have a predictable chloroplast leader peptide. Chloroplasts perform essential cellular functions, directing biosynthesis and gene expression. Despite their importance for cell function and development, the functions of many proteins in the chloroplast are currently poorly understood. Therefore, chloroplast proteomics has received increasing attention.

Fig. 1. Schematic explanation of the proteomics strategy for systematic analysis of the lumenal and peripheral thylakoid proteins. (Peltier JB, et al., 2000)

Our Services

Based on our efficient protocol for the preparation of high-purity organelles or organelle subfractions, our scientists have successfully developed an efficient method to extract chloroplast proteins in plants, and employ a variety of advanced mass spectrometry techniques to identify protein species and membrane composition in chloroplasts. In addition, we provide services for the protein localization and function of chloroplast protein post-translational modifications, as well as chloroplast protein import and other chloroplast biology research. Our chloroplast proteomics services include but are not limited to:

• Chloroplast Proteins Extraction
• Chloroplast Proteome Analysis Services

Methods for Chloroplast Proteomics Research

Our method is characterized by high throughput, high sensitivity, high accuracy, high resolution, low sample consumption, and more information. At the same time, we combine advanced technology with various analysis software or databases to provide you with reliable proteomics analysis services.

• Two-dimensional gel electrophoresis technique.
• Two-dimensional fluorescence difference gel electrophoresis technique.
• Matrix-assisted laser desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS).
• Tandem mass spectrometry.
• In silico methods based on the plastid proteome.
• Targeted approaches and functional proteomics.

Lifeasible provides you with professionally and economically oriented chloroplast engineering service projects. Each project will be individually designed to meet our customers' requirements and described in a detailed project report. Our customers have direct access to our experts and provide immediate feedback on any online inquiries. If you are interested in our services, please do not hesitate to contact us.

References

1. Peltier JB, Friso G, Kalume DE, et al. (2000) Proteomics of the chloroplast: systematic identification and targeting analysis of lumenal and peripheral thylakoid proteins. Plant Cell. 12(3):319-341.
2. Baginsky S, Gruissem W. (2004) Chloroplast proteomics: potentials and challenges[J]. Journal of Experimental Botany. 55(400): 1213-1220.