Regulation of Chloroplast Metabolism

Photosynthesis is the process of converting solar energy into chemical energy and inorganic matter into organic matter. Due to the continuous improvement of in vitro chloroplast separation technology, it was found that in addition to converting light energy into bioenergy and fixing CO₂, chloroplasts cooperate with other parts of green cells to reduce nitrate, nitrite, sulfate, and synthesize starch. , sucrose, amino acids, proteins, organic acids, lipids, nucleic acids and other substances. The chloroplast serves as a site for photosynthetic reactions and has the function of providing metabolic intermediates that support plant growth. The three most common environmental stresses (temperature, light, and osmotic stress) affect chloroplast energy transduction in multiple ways. In addition, redox regulation of chloroplasts is also involved in regulating early stages of plant development and responses to environmental stresses. Plants have evolved a tremendous ability to reshape their metabolism in response to short- and long-term environmental perturbations.

Fig. 1. Chloroplast redox regulatory pathways in the light and the dark. (Cejudo F J, et al., 2021)

Services

Plant growth must cope with persistent and unpredictable changes in environmental conditions, and chloroplasts have important functions as sensors of environmental conditions and as a source of retrograde signals that coordinate plant growth at different developmental stages. Over the years, the team at Lifeasible has accumulated extensive experience in chloroplast metabolomic analysis, focusing on the regulation of chloroplast energy conversion and storage pathways. Our engineers are committed to providing chloroplast metabolism regulation services to customers, analyzing the regulation of chloroplast metabolism in response to temperature, illumination and osmotic stress. Furthermore, we tune chloroplast performance according to environmental factors by analyzing the redox network of chloroplasts. We will choose the best chloroplast metabolism regulation strategies for you:

· Temperature
Changes in growth temperature trigger long-term adaptive responses that rebalance responses to promote efficient metabolism. We predict metabolic changes in response to temperature adaptation by building computational models.

· Illumination
The quantity and quality of light interacts directly with the light reaction of photosynthesis. We phosphorylated PSⅡ light-harvesting complex (LHCⅡ) and acetylated the thylakoid protein NSI to analyze plant photosynthesis under dynamic light conditions.

· Osmotic Stress
Osmotic stress in plants can arise from soil salinity or leaching. We analyzed chloroplast osmoregulation by post-translational modification of the envelope K⁺ exchange antiporters (KEA1 and KEA2) acetylated.

Competitive Advantages

• Professional team with rich experience in chloroplast photosynthesis and metabolic analysis
• Supports the important role of post-translational modifications for dynamic photosynthesis.
• Linking the regulation of chloroplast metabolism to light, temperature and osmotic stress.
• Advancing understanding of chloroplast redox regulation and chloroplast primary metabolism.
• Fully customized service, providing professional and detailed analysis of results.
• Providing new ideas for enhancing photosynthesis under adverse environmental conditions.

Lifeasible can meet the needs of customers on time and on budget through a wide range of chloroplast metabolomics analysis strategies. Our aim is to be customer-centric and to provide the highest quality service to customers around the world. Our skilled and dedicated scientific researchers ensure that the most appropriate methods and techniques are selected for each specialized chloroplast project. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

References

1. Cejudo F J, González M C, Pérez-Ruiz J M. (2021) Redox regulation of chloroplast metabolism[J]. Plant Physiology. 186(1): 9-21.
2. Armbruster U, Strand D D. (2020) Regulation of chloroplast primary metabolism[J]. Photosynthesis Research. 145(1): 1-3.