Rubisco Large Subunit, form I (rabbit) Antibody

Form	Lyophilized in PBS pH 7.4
Quantity	50 µL
Reconstitution	For reconstitution add 50 µl of sterile water
Applications	Immunofluorescence/confocal Immunolocalization (IL) (IF), Immunogold (IG), Tissue Printing (TP), Western Blotting (WB)
Advantages	This antibody is especially suitable for quantifying of Rubisco in plant and algal samples.
Recommended Dilution	Immunofluorescence/confocal microscopy (IF), 1: 1000 (IG), 1: 250 for images see Prins et al. (2008), detailed protocol available on request, 1: 800 (TP), 1: 5000 - 10 000 (WB)
Expected | Apparent MW	52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
Host species	Rabbit
Reactivity	Lichens, algae, cyanobacteria, dinoflagellates, diatoms.
Immunogen	KLH-conjugated synthetic peptide conserved across all known plant, algal and cyanobacterial RbcL protein sequences (form I L8S8 and form II L2), including, Arabidopsis thaliana, Hordeum vulgare, Oryza sativa, Chlamydomonas reinhardtii, Synechococcus PCC 7920.
Additional Information	Anti-RbcL is suitable for a cellular compartment marker of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles.
Storage	Store at -20°C.
Storage Comment	Store lyophilized/reconstituted at -20°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles, please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Purity	Serum
Clonality	Polyclonal Antibody