Rubisco Large Subunit, form I (rabbit) Antibody

Form	Lyophilized
Quantity	50 µL
Reconstitution	For reconstitution add 50 µl of sterile water
Applications	Immunofluorescence/confocal Immunolocalization (IL) (IF), Immunogold (IG), Tissue Printing (TP), Western Blotting (WB)
Advantages	As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody together with Agrisera Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples.Example of a simulataneous Western Blotting detection with RbcL, PsbA and PsaC antibodies.
Recommended Dilution	Immunofluorescence/confocal microscopy (IF), 1: 1000 (IG), 1: 250 for images see Prins et al. (2008), detailed protocol available on request, 1: 800 (TP), 1: 5000 - 10 000 (WB)
Expected | Apparent MW	52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
Host species	Rabbit
Reactivity	Lichens, algae, cyanobacteria, dinoflagellates, diatoms.
Immunogen	KLH-conjugated synthetic peptide conserved across all known plant, algal and cyanobacterial RbcL protein sequences (form I L8S8 and form II L2), including, Arabidopsis thaliana O03042, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5.
Additional Information	Anti-RbcL is suitable for a cellular compartment marker of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles. As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants.
Storage Comment	Store lyophilized/reconstituted at -20°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles, please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Purity	Serum
Clonality	Polyclonal Antibody