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Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2+ fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
Form | Liquid in PBS pH 7.4, conjugated to ALP |
Quantity | 40 µg |
Applications | ELISA (ELISA), Western Blotting (WB) |
Recommended Dilution | 1 : 5000 (ELISA), 1 : 10 000-25 000 (WB) |
Expected | Apparent MW | 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii) |
Host species | Rabbit |
Reactivity | Lichens, algae, cyanobacteria, dinoflagellates, diatoms. |
Immunogen | KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana AtCg00490, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5 |
Additional Information | Anti-RbcL is suitable for a cellular compartment marker of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31,25 fmoles, As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. |
Storage | Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. |
Shelf-Life | 12-18 months |
Purity | Antigen affinity purified serum |
Clonality | Polyclonal Antibody |