Rubisco Large Subunit, form I (chicken) Antibody

Form	Liquid
Quantity	50 µL
Applications	Immunogluorescence (IF), Immunolocalization (IL), ImmunoGold (IG), Western Blotting (WB)
Recommended Dilution	(IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 µg of total cellular protein, (WB)
Expected | Apparent MW	52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
Host species	Chicken
Reactivity	Arabidopsis thaliana, Fragilariopsis cylindrus, Lobaria pulmonaria, Medicago sativa, mixed phytoplankton, Microcystis aeruginosa PCC7806, Pinus strobus, Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcus sp. PCC7842, Thiobacillus sp. Ulmus sp.
Immunogen	KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana
Additional Information	This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments.
Storage	Store at 4°C; make aliquots to avoid working with a stock, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes
Purity	Total IgY in PBS pH 8.0+ 0.02% sodium azide
Clonality	Polyclonal Antibody