Rubisco Large Subunit, form I (affinity purified) Antibody

Form	Lyophilized in PBS pH 7.4
Quantity	50 µg
Reconstitution	For reconstitution add 50 µl of sterile water
Applications	Western Blotting (WB)
Advantages	As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody together with Agrisera Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples.
Recommended Dilution	1 : 5000-10 000 (WB)
Expected | Apparent MW	52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
Host species	Rabbit
Reactivity	Lichens, algae, cyanobacteria, dinoflagellates, diatoms.
Immunogen	KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana, Hordeum vulgare, Oryza sativa, Chlamydomonas reinhardtii, Synechococcus PCC 7920.
Additional Information	Anti-RbcL is suitable for a cellular compartment marker of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles.
Storage	Store at -20°C.
Storage Comment	Store lyophilized/reconstituted at -20°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles, please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Purity	Affinity purified serum
Clonality	Polyclonal Antibody