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Anti-RbcL is suitable for a cellular compartment marker of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles.
Form | Lyophilized in PBS pH 7.4 |
Quantity | 50 µg |
Reconstitution | For reconstitution add 50 µl of sterile water |
Applications | Western Blotting (WB) |
Advantages | As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody together with Agrisera Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples. |
Recommended Dilution | 1 : 5000-10 000 (WB) |
Expected | Apparent MW | 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii) |
Host species | Rabbit |
Reactivity | Lichens, algae, cyanobacteria, dinoflagellates, diatoms. |
Immunogen | KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana O03042, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5. |
Additional Information | Anti-RbcL is suitable for a cellular compartment marker of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles. As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. |
Storage Comment | Store lyophilized/reconstituted at -20°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles, please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
Purity | Affinity purified serum |
Clonality | Polyclonal Antibody |