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Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups.
Form | Lyophilized |
Reconstitution | For reconstitution add 100 μL of sterile water. |
Applications | Western Blottingting (WB) |
Comment | In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions. |
Recommended Dilution | 1: 5000 with standard ECL (WB) |
Expected | Apparent MW | 16 kDA |
Host species | Rabbit |
Reactivity | Arabidopsis thaliana, Manihot esculenta, Spinach, Zea mays |
Immunogen | His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060 |
Storage | Store at -20°C. |
Storage Comment | Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
Purity | Serum |
Clonality | Polyclonal Antibody |
Target | PrxQ (PRXQ) |
Handling Advice | Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. |