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Translocon at the Outer Envelope Member of Chloroplasts - Toc consists of three components: Toc34, Toc86, and Toc75. Toc 75 forms an outer membrane porin that may serve as the outer membrane proteinconducting channel.
Form | Lyophilized |
Reconstitution | For reconstitution add 50 μL of sterile water. |
Applications | Immunoprecipitation (IP), ELISA, Western Blottingting (WB) |
Recommended Dilution | 1 : 100 000 with alkaline phosphatase (WB) |
Expected | Apparent MW | 29 kDa |
Host species | Chicken |
Specificity | C-Term |
Reactivity | Pisum sativum, Spinach |
Immunogen | Soluble domain of Pisum sativum Toc34 overexpressed in E.coli and purified by His-tag affinity purification. Overexpressed Toc34 is lacking the transmembrane C-terminal domain. |
Buffer | PBS pH 8.0 + 0.02 % sodium azide |
Storage | Store at -20°C. |
Storage Comment | Store lyophilized/reconstituted at -20°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles, please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
Purification | affinity purified |
Clonality | Polyclonal Antibody |
Isotype | IgY |
Target | Chloroplast Outer Envelope Membrane Translocon Complex Protein (Toc34) |
Handling Advice | Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. |