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This kit provides an efficient procedure to obtain intact chloroplasts from plant leaves.
Pack Size | 1 Kit |
Applications | Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies. |
Reactivity | Plant |
Benefits | 1. Saves time using easy to follow simple and proven protocols. 2. Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting. 3. Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications. |
Kit Components | 1. Chloroplast Isolation Buffer 5 X (CIB) 500 mL. 2. Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 g. 3. Chloroplast Isolation Buffer 5 X (CIB) 4 ea. |
Suitability | The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco. |
Storage | Store at 2-8°C. |
Shelf-Life | 12 months |
Shipping Conditions | Wet Ice |
Handling Advice | Procedures include mechanical cell wall and membrane disruption, removal of cellular debris and leaf tissue by filtration, collection of total cellular chloroplast fractions by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a gradient. |